Intro to Plant Tissue Culture

A beginner-friendly guide to starting your journey with organic plant tissue culture. Learn how to propagate plants in a clean, sustainable way—from setting up a workstation to seeing your first plantlets grow.

Step-by-Step Starter Guide

  1. 1
    Create a clean workspace: Use a simple table in a draft-free area. Wipe all surfaces with diluted alcohol or vinegar.
  2. 2
    Prepare your growth medium: Use a natural, organic culture medium suited for your plant species. Sterilize it properly in jars.
  3. 3
    Select a healthy plant: Choose disease-free mother plants. Young shoots and buds work best for beginners.
  4. 4
    Take your explant: Cut a small shoot tip, leaf, or node using sterilized scissors. Minimize exposure to open air.
  5. 5
    Transfer to the medium: Gently place the plant tissue on the surface of the medium in a clean jar. Seal the jar.
  6. 6
    Provide the right conditions: Keep jars at room temperature with indirect light. Avoid direct sun and temperature extremes.
  7. 7
    Observe and wait: Within a few weeks you’ll see callus or tiny shoots forming. Patience is key!
  8. 8
    Acclimate your plantlets: Once plantlets have roots, open jars gradually to let them adjust to normal air before planting in soil.

🌱 Pro Tip

Small, clean, and patient: Start with just a few jars, keep everything clean, and avoid overhandling your cultures. Simplicity and hygiene are more important than fancy equipment.

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